Journal: iScience

Article Title: Expression of nonmuscle myosin IIC is regulated by non-canonical binding activity of miRNAs

doi: 10.1016/j.isci.2023.108384

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Article Snippet: SureBeads Protein G Magnetic Beads , BioRad , Cat# 1614021.

Techniques: Recombinant, Magnetic Beads, Luciferase, Quantitation Assay, Isolation, Plasmid Preparation, Software

Journal: iScience

Article Title: Immunomodulatory potential of in vivo natural killer T (NKT) activation by NKTT320 in Mauritian-origin cynomolgus macaques

doi: 10.1016/j.isci.2022.103889

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Article Snippet: Bio-Plex Manager Software v6.2 , Bio-Rad, Hercules, CA , Cat#171STND05.

Techniques: Recombinant, Staining, Luminex, Transfection, Stable Transfection, Software

Journal: The Journal of general virology

Article Title: Hepatitis B virus X protein differentially affects the ubiquitin-mediated proteasomal degradation of beta-catenin depending on the status of cellular p53.

doi: 10.1099/vir.0.82836-0

Figure Lengend Snippet: Fig. 3. HBx induces ubiquitination of b-catenin in p53-positive HepG2 cells. (a) HepG2 cells were transfected with either an empty vector or HBX3 (0.5 mg) and analysed by RT-PCR to compare b-catenin RNA levels. (b) HepG2 cells were transiently transfected with either empty vector or HBX3 (0.5 mg) and either mock-treated or treated with 100 mM MG132 for 2 h before harvesting. (c) Increasing amounts of HBX3 were co-transfected with pHA-ubiquitin (5 mg) into HepG2 cells. Ubiquitin- conjugated products were immunoprecipitated with anti-HA antibody as described in Methods. Levels of ubiquitin-attached b- catenin were detected with anti-b-catenin antibody. As a loading control, the amount of IgG in the precipitates and levels of c- tubulin in the total lysates are shown. Each band was quantified with the use of BIOPRORIL BIO 1D image analysis software (Vilber Lourmat) to show the correlation between total and ubiquitinated b-catenin (right).

Article Snippet: Membranes were incubated with anti-b-catenin antibody (#610153; BD Transduction Laboratories); anti-Siah-1, anti-c-myc and anti-p53 antibodies (sc-5505, sc-40 and sc-126, respectively; Santa Cruz Biotechnology); anti-HA antibody (#11 583 816 001; Roche) and anti-tubulin antibody (T-6557; Sigma) and subsequently with the appropriate horseradish peroxidase-conjugated secondary antibodies: goat anti-mouse IgG(H+L)–HRP (#170-6516; Bio-Rad) and rabbit anti-goat IgG–HRP (sc-2768; Santa Cruz Biotechnology).

Techniques: Ubiquitin Proteomics, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Control, Software